Figure 3.
(A) Alignment of predicted amino acid sequences for the selected anti-GST clones. The nucleotide sequences of the VH and VL inserts of eight dominant and three minority clones were determined and translated into protein sequence (single-letter code). Unique VL (i–iii) and VH (i–iv) are shown for the dominant clones. Each minority clone expressed unique VL and VH sequence patterns. The sequences are segregated into CDR and FR regions based on homology to known bovine sequences (11–18). A dash (–) indicates identity to the uppermost sequence; ∗ indicates no amino acid at this position. (B) VL and VH chain usage in the dominant selected anti-GST clones. Three VL (i–iii) and four VH (i–iv) sequences were found in various combinations in the eight dominant clones. Clones G67, G72, G74, and G76 expressed the same VL and VH combination and are therefore identical. Clone G63 differs by only two VH FR residues from clone G77.