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Effect of pressure elevation on mitochondrial potential. Tracings are quantifications of TMRM fluorescence from a single capillary. Capillaries were first loaded with TMRM, then given saponin (0.01%) infusion to permeabilize EC membranes. PLA was maintained at 5 cmH2O (light line) or 15 cmH2O (bold line). Response to FCCP (2 μM) was obtained at PLA of 5 cmH2O. Traces represent data replicated in four capillaries.
