Table 1.

Regulation of costimulatory molecule expression on CD3ɛ-activated T cells by defensins

Costimulatory molecule	Splenic T cells		Peyer’s patch T cells
	10 ng/ml	100 ng/ml	10 ng/ml	100 ng/ml
IL2R	73 ± 5.0%*	125 ± 12.3%*	22 ± 1.3%	48 ± 3.4%*
CD28	65 ± 3.9%*	98 ±  9.7%*	29 ± 2.2%	57 ± 2.7%*
CTLA4	(12 ± 0.8%)	(27 ±  1.5%)	(15 ± 0.9%)	(36 ± 2.4%)
CD40L	(4 ± 0.3%)	(13 ±  0.7%)	(34 ± 1.7%)	(42 ± 3.1%)*

CD3ɛ-activated splenic and Peyer’s patch T cells from naive mice were incubated with 0, 10, and 100 ng/ml of HNPs. The percent increase (or decrease) of the costimulatory molecule expression on CD3ɛ-activated T cells was calculated as the percent of double positive CD3⁺ and IL2R⁺, CD28⁺, CTLA-4, or CD40L⁺ cells in cultures containing HNPs minus the percent gated of double positive cells in cultures without HNPs divided by the latter. Studies were repeated three times, and the data presented are the mean percent change ± SEM of these experiments. 

^*Statistically significant differences (P < 0.05) relative to cultures without HNPs.