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. 1999 Jan 19;96(2):657–662. doi: 10.1073/pnas.96.2.657

Figure 2.

Figure 2

EPR spectra of NO⋅ formation from cardiac SR vesicles in the presence of a Fe(MGD)2 complex under various conditions for 60 min at room temperature. Fe(MGD)2 only (A); + SOD in condition A (B); + a mixture of NOS substrates and cofactors in condition B (C); + cardiac vesicles in condition C (D); + 7-NI in condition D (E); and + d-arginine in the presence of vesicles and cofactors (F) (see Methods for the final concentrations of the reaction components). These results show that 7-NI sensitive, endogenous NO⋅ (a triplet spectrum) is generated by the isolated cardiac SR vesicles. The data are presented from one of four similar independent experiments in each case.