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. 2003 Feb 15;111(4):453–461. doi: 10.1172/JCI15924

Figure 1.

Figure 1

aP2-Pref-1/hFc transgene expression. (a) Analysis of transgene expression by Northern blot analysis in 3AS line of aP2-Pref-1/hFc mice. Total mRNA was extracted from the tissues of 10-week-old transgenic mice (TG) and wild-type littermates and probed with radiolabeled Pref-1 cDNA probe. S, spleen; Th, thymus; Li, liver; Lu, lung; K, kidney; SM, skeletal muscle; BAT, brown adipose tissue; Rep, reproductive fat pad; Ing, inguinal fat pad; Ren, renal fat pad. (b) Western blot analysis for Pref-1/hFc fusion protein in serum of three lines (1AS, 2AS, and 3AS) of aP2-Pref-1/hFc transgenic mice. The serum proteins were separated by SDS-PAGE and probed with human Fc antibody. The antibody detected the 75-kDa of Pref-1/hFc fusion protein in three founder lines of transgenic but not in wild-type mice.