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. 2003 Feb 15;111(4):515–527. doi: 10.1172/JCI16079

Figure 1.

Figure 1

Effect of acute exposure to albumin on IL-8 expression by PTECs. (a) Time response. Confluent, growth-arrested cells were cultured in serum-free medium or medium containing 5 mg/ml HSA for 3–24 h. IL-8 gene expression (line) was quantified by RT-PCR, while protein secretion in culture supernatants (bars) was assayed by ELISA. Results are means ± SD obtained from duplicate experiments of three different kidney preparations. *P < 0.0001, †P = 0.008, #P = 0.027, **P = 0.002, ‡P = 0.004, §P = 0.01 compared with growth-arrested cells incubated in serum-free medium alone for equivalent duration. (b) Dose response. Confluent, growth-arrested cells were cultured in serum-free medium or medium containing escalating doses of HSA for 3 h. IL-8 gene expression (line) and protein secretion (bars) were measured as stated above. Results are means ± SD obtained from duplicate experiments of three different kidney preparations. *P = 0.011, † P = 0.004, ‡P = 0.001, §P < 0.0001, #P = 0.002 compared with growth-arrested cells incubated in serum-free medium alone.