Figure 12.

Immunohistochemical staining for IL-8 and infiltrating leukocytes. (a and b). Representative experiment from a non-nephrotic control subject, showing background staining only. (c and d) Representative staining from a nephrotic subject, demonstrating the predominance of tubular over glomerular staining for IL-8 protein. (e and f) IL-8 staining in tubules of a representative nephrotic subject followed a focal pattern of distribution. Staining of the section with anti-CD44 mAb (g and h) demonstrated infiltration by CD44-positive leukocytes of the interstitial space in the proximity of the IL-8–expressing tubules. (a, c, e, g) ×100; (b, d, f, h) ×200 of marked area in the corresponding left-hand panels, all counterstained with hematoxylin.