Figure 13.
Histologic score for IL-8 immunostaining in renal tubules. (a) Scoring in non-nephrotic versus nephrotic subjects. Immunohistochemical staining for tubular IL-8 antigen was performed on renal biopsy specimens obtained from 25 patients with nephrotic range or minimal proteinuria and graded in an observer-blinded manner on an arbitrary scale of 0–3 (see text for scoring criteria). Circles and horizontal lines are individual scores of each patient and mean scores in each group, respectively. Horizontal bars and vertical lines are means ± SE of daily urinary protein excretion in each group. (b) Correlation between the magnitude of proteinuria and staining score for IL-8 in renal tubules of 18 nephrotic subjects by linear regression (r2 = 0.332, b = 0.116 [95% confidence interval, 0.029–0.203], P = 0.012).