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. 2002 Nov;184(22):6235–6249. doi: 10.1128/JB.184.22.6235-6249.2002

FIG. 2.

FIG. 2.

Construction of a tum promoter-lacZ reporter construct. (A) The control region of the Fels-2 tum gene was PCR amplified and cloned adjacent to the lacZ gene of the Topo cloning vector (Stratogene). Strains with this high-copy-number plasmid show constitutive lacZ expression. The tum control region was amplified from the above plasmid (B) and inserted (by recombination following linear transformation) adjacent to the lacZ gene of the low-copy-number plasmid F′128 (C). Strains with this plasmid show LexA-controlled expression of LacZ.