TABLE 6.

Sequenced damage-inducible (din) gene insertions

Damage-inducible gene insertiona	Gene containing the MudJ insertionb	Location of identified gene	Repression by LexA overexpressionc	Inducibility by mitomycin Cd
				lexA+	lexA(Ind−)
din-240::MudJ	recE	Prophage Gifsy-2e	Weak	Yes	None
din-11::MudJ (identical to din-80::MudJ)	recE	Prophage Gifsy-2e	Weak	Yes	±
din-14::MudJ	Gene for putative T4-like terminase large subunit	Prophage Gifsy-2	ND	Yes	None
din-243::MudJf	Gene resembling the H gene of phage lambda (tail component)	Prophage Gifsy-2	Strong	Yes	None
din-10::MudJ	ORF between genes that resemble the J and tail fiber genes of phage lambda	Prophage Gifsy-2	Strong	Yes	None
din-9::MudJ	Between genes that resemble the “gpshp” and gp-7 genes of phage 21	Prophage Gifsy-1	Strong	Yes	None
din-84::MudJ	ybhA	Chromosome, 18.3 min	Weak	Yes	None

^aIsolated by Smith et al. (48).

^bDetermined by single-primer PCR and sequencing (see Materials and Methods). ORF, open reading frame.

^cData from Smith et al. (48). ND, not done.

^dDetermined by replica plating duplicate patches of strains to NB-X-Gal plates containing 0, 50, 100, 150 and 200 ng of mitomycin C per ml.

^eThe recE genes of Gifsy-1 and -2 are identical, and therefore linkage (or lack thereof) with an nadB insertion (nadB215::Tn10) approximately 11 kb from recE in Gifsy-1 was used to determined if the recE insertions were in Gifsy-1 or -2.

^fWith no DNA damage and a wild-type LexA protein, this gene is fully repressed; in a lexA(Ind⁻) background, the gene is transcribed at a low level but does not appear to be upregulated in the presence of DNA damage.