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Human recombinant LH-RH-Rc protein expression in response to ecdysone analogs. After 24-h induction with ecdysone analog at the indicated concentration, 100 μg of cell lysate was fractionated on 6% PAGE and transferred onto poly(vinylidene difluoride) membrane for Western analysis. Western blotting was performed with monoclonal primary antibody to hemagglutinin tag of LH-RH-Rc. Actin blots show no statistical difference between amounts of protein loaded in any two groups.
