Figure 2.

Luciferase activity in LH-RH-Rc stable expressing cells induced by [d-Lys⁶] LH-RH (mean ± SEM). Stable expressing LH-RH-Rc cells were transiently transfected with 1 μg α-846 Luc/paLuc, and the receptor was induced with 10 μM ecdysone analog for 24 h. [d-Lys⁶] LH-RH agonist and/or LH-RH antagonist (Cetrorelix) then were added at the indicated concentrations to triplicate wells of transiently transfected cells. After 24 h, cells were lysed in 150 μl of lysis buffer, and luciferase activity was assayed in 100 μl of cell lysate according to vendor. ∗, P < 0.005; ∗∗, P < 0.0001, significant difference from the value of control group, by Student’s t test.