TABLE 2.
Primers used to construct the hk11 and rr11 deletion mutants by PCR restriction-ligation mutagenesis
| Primer | Nucleotide sequence (5′ → 3′)a | Amplicon (bp) |
|---|---|---|
| HK11-P1 | CTGAAGGAAGCCTTATGCG | 763 |
| HK11-P2 | GGCGCGCCCCGGAAATGATGGTAACTGCCG | |
| HK11-P3 | GGCCGGCCAAGTGCTGAAAAGGGGG | 666 |
| HK11-P4 | CATAGACAACGGCTTGGGTC | |
| RR11-P1 | AACGCCACCTCAACCAAA | 887 |
| RR11-P2 | GGCGCGCCGCCTCACCGATAACTTCTACATT | |
| RR11-P3 | GGCCGGCCCCTTCAGCATCATTTAGTGCCAC | 565 |
| RR11-P4 | CACCCACATCCTCAAAGGTC | |
| Em cst-P1 | GGCGCGCCCCGGGCCCAAAATTTGTTTGAT | 860 |
| Em cst-P2 | GCTGGCCGGCCAGTCGGCAGCGACTCATAGAAT |
a
AscI sites are in boldface; FseI sites are underlined.