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. 2002 Nov;184(22):6207–6215. doi: 10.1128/JB.184.22.6207-6215.2002

FIG. 1.

FIG. 1.

Purification of flagellin of H. halophilus and specificity of the antiflagellin antiserum. Flagella were prepared as described in Materials and Methods and subjected to SDS-PAGE (lane 1). The 47-kDa protein was electroeluted (lane 2) and identified as flagellin by its N-terminal amino acid sequence. The specificity of the generated antiserum was determined in a Western blot analysis using crude cell extract of H. halophilus (lane 3).