FIG. 3.

Quantification of the Cl⁻ dependence of the cellular flagellin level of H. halophilus. Cells were grown under isosmotic conditions (the salt concentration of the medium was kept constant at 1 M by the appropriate addition of NaNO₃) with the Cl⁻ concentrations indicated. Cells were grown to the late exponential growth phase and harvested, and 25 μg of protein was applied to an SDS-polyacrylamide gel. The flagellin concentration was determined by Western blot analysis (A), and the signal intensity was plotted against the Cl⁻ concentration (B).