RT-PCR analysis of lysozyme-like gene expression in clinically isolated H. pylori strains. By using the vacA gene as an internal standard, lysozyme-positive strains HPTS142, HPTS65, and HPTS36 (lanes 1 to 3) and lysozyme-negative strains HPTS12, HPTS50, and HPTS90 (lanes 4 to 6) were analyzed for gene expression. Additional details are described in Materials and Methods.