FIG. 3.

Zymogram analysis of H. pylori protein extracts. Portions (10 μg) of total proteins extracted from H. pylori strains were loaded on an SDS-12% polyacrylamide gel containing 0.05% (wt/vol) M. lysodeikticus cells. After electrophoresis, the gel was incubated in renaturation buffer overnight and stained with 0.1% methylene blue. Lanes 1 and 2, crude protein extracts from lysozyme-negative strains HPTS12 and HPTS90, respectively; lanes 3, 4, 5, and 6, crude protein extracts from lysozyme-positive strains HPTS142, HPTS65, HPTS61, and HPTS36, respectively; lane 7, bovine serum albumin and ovalbumin (2 μg). The positions of molecular weight (MW) markers are indicated on the left.