FIG. 2.

Cloning of arcC and the surrounding genes in E. faecalis and gene organization of the sequenced region. The bars at the top illustrate to scale the inserts in isolated λgt11 clones, indicating the positions of arcA (striped arrow) and arcC (solid arrow) and the orientation of the inserts with respect to the flanking forward (F) and reverse (R) sequences of the cloning site in λgt11. Insert-specific primers used to characterize the constructions are shown as solid arrowheads and are named as described in Materials and Methods. The broken vertical lines give relevant restriction sites, some of which were used for subcloning of fragments r, a, b, and c (double-headed arrows). The bottom arrows schematize the gene organization of the two strands of the sequenced region, with indications of the number of amino acid (aa) residues expected for each gene product. The positions of the six predicted stem-loops (see Materials and Methods) are indicated by the open circles.