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. 1999 Jan 19;96(2):674–679. doi: 10.1073/pnas.96.2.674

Figure 2.

Figure 2

Regulation of preproinsulin mRNA expression by leptin in INS-1 β-cells. (A) Southern detection of OB-RB RT-PCR products in INS-1 cells and rat hypothalamus (positive control). (B) Representative Northern blot for rat preproinsulin mRNA in INS-1 cells treated for 6 h and 16 h with leptin (0.6 or 6.25 nM) or vehicle at 25 mM glucose. (C) Representative Northern blot for rat preproinsulin mRNA in INS-1 cells treated for 16 h with 6.25 nM leptin or vehicle at 11.1 mM glucose in the absence or presence of 10 nM GLP-1. For each study condition, the experiments have been performed at least twice.