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. 2002 Nov;184(22):6138–6145. doi: 10.1128/JB.184.22.6138-6145.2002

FIG. 2.

FIG. 2.

Immunogenicity and iron-regulated expression of the 45-kDa protein. (A) Lanes contain whole-cell lysates prepared from the following gonococcal strains: 1, FA19 (TbpA+ TbpB+); 2, FA6747 (TbpA− TbpB+); 3, FA6905 (TbpA+ TbpB−); and 4, FA6815 (TbpA− TbpB−). Strains were grown under iron-replete (+) and iron-stressed (−) conditions as indicated at the top and standardized by cell density. The lane labeled AP contains affinity-purified proteins that served as immunogens to generate antiserum with which the blot was probed. Molecular size standards (in kilodaltons) are indicated on the right, and the locations of relevant proteins are indicated on the left. The immunoblot was probed with polyclonal antiserum raised against affinity-purified proteins isolated from FA19. (B) Immunoblot was probed with polyclonal antiserum raised against affinity-purified proteins isolated from FA6905. Lanes are labeled as indicated for panel A.