FIG. 2.

Induction of β-galactosidase activity in sig1 (AKS10) and sig2 (AKS11) mutant and wild-type (R1) strains carrying a plasmid-borne lacZ transcriptional fusion to groESL (A) or dnaKJ (B). Heat shock protocol was as for Fig. 2, except the temperature was shifted to 40°C. Duplicate samples were removed at 0, 30, 60, and 120 min postinduction to assess β-galactosidase activity. Error bars represent standard deviations from the averages of three experiments. Error bars for 30°C background data points overlap (not shown).