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Expression of PhaR in E. coli BL21(DE3). Proteins were separated by SDS-PAGE. Lane 1, total proteins of E. coli carrying the plasmid with phaR before induction by IPTG; lanes 2 to 5, total cellular proteins of E. coli carrying the plasmid with phaR after 1, 2, 3, and 4 h of induction by IPTG, respectively. The arrow denotes the PhaR band. Numbers at left are molecular masses in kilodaltons.
