TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype or description |
|---|---|
| Strains | |
| LT2 | Wild type |
| TT248 | hisC527(Am)cysA1348(Am) |
| DM2 | purH355 |
| DM42 | purI2152::MudJ |
| DM521 | zfb-8017::Tn10d(Tc)apurI2937 |
| DM531 | zfb-8017::Tn10d(Tc) purI2944 |
| DM539 | zfb-8017::Tn10d(Tc) purI2945 |
| DM929 | purF2085 purE884::Tn10d(Tc) |
| DM1216 | zfb-8017::Tn10d(Tc) purI2944 zje-8058::MudJbalaW221 |
| DM1472 | zfb-8017::Tn10d(Tc) purI3004 |
| DM1473 | zfb-8017::Tn10d(Tc) purI3005 |
| DM1474 | zfb-8017::Tn10d(Tc) purI3006 |
| DM1476 | zfb-8017::Tn10d(Tc) purI3008 |
| DM1479 | zfb-8017::Tn10d(Tc) purI3011 |
| DM1481 | zfb-8017::Tn10d(Tc) purI3013 |
| DM1482 | zfb-8017::Tn10d(Tc) purI3014 |
| DM1483 | zfb-8017::Tn10d(Tc) purI3015 |
| DM1936 | purF2085 |
| DM2093 | zfb-8017::Tn10d(Tc) purI3006 zxx-8087::MudJ sup-5851 |
| DM2095-DM2102 | zfb-8017::Tn10d(Tc) purI2944 alaW231-alaW238 |
| DM2625 | zfb-8017::Tn10d(Tc) purI3006 sup-5852 |
| DM2626 | zfb-8017::Tn10d(Tc) purI3006 sup-5853 |
| DM2627 | zfb-8017::Tn10d(Tc) purI3006 sup-5854 |
| DM2628 | zfb-8017::Tn10d(Tc) purI3006 sup-5855 |
| DM2629 | zfb-8017::Tn10d(Tc) purI3006 sup-5856 |
| DM2630 | zfb-8017::Tn10d(Tc) purI3006 sup-5857 |
| DM2631 | zfb-8017::Tn10d(Tc) purI3006 sup-5858 |
| DM2633 | zfb-8017::Tn10d(Tc) purI3006 sup-5859 |
| DM5976 | purH355 purE884::Tn10d(Tc) |
| DM6068 | purH355 hisG1102 |
| DM6069 | purH355 |
| DM6123 | purG2324::MudJ zxx9126::Tn10d(Tc) stm4068-1 purH355 |
| DM6124 | purG2324::MudJ zxx9126::Tn10d(Tc) stm4068-1 |
| Plasmids | |
| pJZ531 | Cmr 1.3-kb insert with alaW in HindIII site of pSU19 |
| pJZala1 | Cmr 0.6-kb insert containing alaW in HindIII/SmaI of pSU19 |
| pJZG252A | Cmr 1.2-kb insert of G252A purI gene in SmaI site of pSU19 |
| pLUX1c | Cmr wild-type luxAB genes in pCAM203 |
| pLUX2c | Cmr includes an amber codon at position 13 of luxB gene |
| pLUX3c | Cmr includes an ochre codon at position 13 of luxB gene |
| pLUX4c | Cmr includes an opal codon at position 13 of luxB gene |