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Plant-produced 38C13 scFv does not contain carbohydrates. PAS stain was used to reveal the total carbohydrate content of 10 μg of 38C13 IgM (lane 1) or 5 and 25 μg of HRP (lanes 2 and 3) but not in 5 or 25 μg of 38C13 scFv made in plants (lanes 4, 5, and 6a). Coomassie staining was done in parallel on lane 6b, which contains 25 μg of 38C13 scFv, as well as on size standards (lane 7, in kDa) as indicated.
