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. 1999 Jan 19;96(2):715–720. doi: 10.1073/pnas.96.2.715

Figure 4.

Figure 4

Fluorescence micrographs of worms fed on PA14/GFP (B), DH5α/GFP (D), and gacA SW7-4/GFP (F) for 48 h. The cellular structures of the same worms visualized under Nomarski phase contrast are shown in A, C, and D, respectively. (B) The entire lumen of worms fed on PA14/GFP was filled with GFP-expressing PA14. PA14/GFP began to accumulate after 36 h of feeding. In contrast, the lumen of worms feed on DH5α/GFP (D) and gacA SW7-4/GFP (F) showed no detectable GFP (see arrows), indicating that very few, if any, of the nonpathogenic bacteria were present. The intestinal cells were highly fluorescent in D and E because of autofluorescence. For gacA SW7-4/GFP, GFP expression was detected at 72 h. The micrographs for PA14/GFP are shown at ×400 magnification, whereas the micrographs for DH5α/GFP and gacA SW7-4/GFP are at ×160 magnification.