TABLE 1.
Strains and plasmids used
| Strain or plasmid | Genotype and/or phenotype | Source or reference |
|---|---|---|
| Escherichia coli strains | ||
| KL98 | Hfr strain, thi Sms | P. Oliver |
| DH5αλpir | λpir lysogen of DH5α for replication of oriR6K, oriT, and mob region of RP4, Kms | N. Judson, GIBCO-BRL |
| Sm10 | Mobilizing strain, transfer genes of RP4 integrated in chromosome | 21 |
| Burkholderia pseudomallei strains | ||
| KHW | Clinical strain | E. H. Yap |
| KHWΔfliCKm | Derivative of KHW, with central fragment of the fliC gene replaced by a 2.3-kb kanamycin cassette from pUTKm | This study |
| Plasmids | ||
| pJQ200mp18 | Suicide vector, oriT and mob region from RP4 and sacB from Bacillus subtilis, Gmr | 19 |
| pJQ200mp18fliCKm | pJQ200mp18 with 3.4-kb kanamycin cassette-disrupted B. pseudomallei fliC sequence inserted at the SmaI site | This study |
| pUTKm | Source of 2.3-kb Tn903-derived Kmr cassette | 4 |
| pUCP28T | Broad-host-range vector; oriT and mob region from RP4, Tpr | H. Schweizera |
| pUCP28TfliC | Contains a 1.5-kb full-length B. pseudomallei fliC gene and promoter inserted into the SmaI site by poly(I) tailing | This study |
a
See reference 27.