TABLE 2.
Determination of bacterial loads in infected with KHW, KHWΔfliCKm, and KHWΔfliCKm /pUCP28TfliCa
| Strain | Organ | Expt | Bacterial load (CFU/organ)
|
|||
|---|---|---|---|---|---|---|
| 2 days after infection | 4 days after infection | 8 days after infection | 14 days after infection | |||
| KHW | Lung | 1 | NTb | 5.5 × 107 | —c | — |
| 2 | NT | 1.4 × 106 | — | — | ||
| 3 | NT | 1.4 × 104 | — | — | ||
| Spleen | 1 | NT | 1.9 × 106 | — | — | |
| 2 | NT | 4.2 × 105 | — | — | ||
| 3 | NT | 4.2 × 104 | — | — | ||
| KHWΔfliCKm | Lung | 1 | 60 | 0 | 0 | NT |
| 2 | 110 | 0 | 0 | NT | ||
| 3 | 0 | 0 | 0 | NT | ||
| Spleen | 1 | 0 | 0 | 0 | NT | |
| 2 | 0 | 0 | 0 | NT | ||
| 3 | 0 | 0 | 0 | NT | ||
| KHWΔfliCKm/pUCP28TfliC | Lung | 1 | 0 | 4.1 × 106 | 2.3 × 103 | 2.2 × 106 |
| 2 | 1.2 × 104 | 1.1 × 104 | 2.9 × 105 | — | ||
| 3 | 5.9 × 104 | 5.2 × 106 | — | — | ||
| Spleen | 1 | 3.7 × 104 | 1.9 × 106 | 4.8 × 106 | 5.0 × 107 | |
| 2 | 3.1 × 104 | 5.4 × 105 | 1.4 × 107 | — | ||
| 3 | 4.9 × 103 | 0 | — | — | ||
a
Ten mice were each intranasally infected with 100 CFU of KHW or KHWΔfliCKm, and 13 mice were infected with 100 CFU of KHWΔfliCKm/pUCP28TfliC.
b
NT, not tested.
c
—, no animals were available for analysis due to death from the disease.