FIG. 3.

Increased CCh-stimulated ion transport responses in infected xenografts are reversed by indomethacin. Xenografts were stripped of their seromuscular layers, infected with Salmonella with or without concurrent treatment with the cyclooxygenase inhibitor indomethacin (10 μM) for 1 h, mounted in Ussing chambers, and allowed to equilibrate for 1 h prior to stimulation with agonists. (A) When xenografts were stimulated with the Ca²⁺-dependent agonist, CCh, ion transport, measured as a decrease in PD, was significantly increased after 2 h of serovar Typhimurium infection compared to controls. ✽, P < 0.05 versus control (n = 5). In tissues that were treated with indomethacin, the Salmonella-induced enhanced secretory responses to CCh were inhibited. #, P < 0.05 versus Salmonella-infected tissue (n = 5). The data shown in panel A represent the incremental changes in PD evoked by CCh addition (i.e., ΔPD). (B) Typical time course of absolute PD response to CCh under the three conditions studied.