FIG. 4.

Increased cAMP-dependent ion transport responses in infected xenografts are reversed by indomethacin. Stripped xenograft tissues were infected with Salmonella with or without concurrent treatment with the cyclooxygenase inhibitor indomethacin (10 μM) for 1 h, mounted in Ussing chambers, and stabilized for 1 h prior to stimulation with agonists. (A) In tissues that were treated with indomethacin the Salmonella-induced enhanced secretory responses to PGE₂ were inhibited. The data are expressed as incremental changes in PD evoked by PGE₂ addition (i.e., ΔPD). ✽, P < 0.05 versus control; #, P < 0.05 versus Salmonella-infected tissue (n = 5). (B) Typical time courses of the absolute PD responses to PGE₂ under the conditions studied. (C and D) Similar results obtained with another cAMP-dependent agonist, forskolin (1 μM). ✽✽, P < 0.01 versus control; #, P < 0.05 versus Salmonella-infected tissue (n = 6).