FIG. 7.

Indomethacin decreases Salmonella-induced PGE₂ secretion into the basolateral medium. Xenografts stripped of seromuscular layers were infected with Salmonella for 2 h in the presence or absence of indomethacin, and apical and basolateral solutions were analyzed for secreted PGE₂ by enzyme-linked immunosorbent assay. Salmonella infection induced a significant increase in basolateral PGE₂ secretion, whereas apical PGE₂ secretion was unaltered compared to control tissues. The enhanced PGE₂ secretory responses to Salmonella were reversed in the presence of 10 μM indomethacin. ✽✽, Significantly greater than corresponding basolateral release in the presence of indomethacin (P < 0.01); ##, significantly greater than basolateral release in uninfected tissue (P < 0.01) (as determined by analysis of variance; n = 5).