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hap homologs in type a H. influenzae. Chromosomal DNA was digested with EcoRI, separated by agarose electrophoresis, transferred to nylon membranes, and hybridized with a hap probe. Lanes 1, 2, 9, and 10, invasive isolates; lanes 3 to 8, noninvasive type a isolates; lane 11, NTHi. Molecular sizes are noted on the left. A second 4-kb hybridizing band is present in lanes 2, 5, 6, and 10, suggesting the presence of two alleles of hap or a second homologous gene in these strains.
