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. 1999 Jan 19;96(2):721–725. doi: 10.1073/pnas.96.2.721

Figure 2.

Figure 2

pH and temperature dependence of racemase activity. (A) Racemase activity was assayed at 37°C in media containing 50 mM Mes-Tris (pH 6.0–6.5), 50 mM Tris⋅HCl (pH 6.8–8.8) of 50 mM CAPS-NaOH (pH 9–10.5), 20 mM l-serine, 100 μg/ml purified enzyme, 1 mM EDTA, 2 mM DTT, and 15 μM PLP. (B) Racemase activity was performed at different temperatures in a medium containing 50 mM Tris⋅HCl, pH 8.0/20 mM l-serine, 100 μg/ml purified enzyme, 1 mM EDTA, 2 mM DTT, and 15 μM PLP. The reaction was stopped after 4 h and analyzed both by chemiluminescence and HPLC assay. The experiment was replicated three times by using different preparations with similar results.