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Absorption spectra of purified serine racemase. Purified enzyme (70 μg/ml) was preincubated for 10 min in medium containing 10 mM KPi (pH 7.2), 2 mM DTT, 1 mM EDTA, and 10 μM PLP, either in the absence (Control) or in the presence of 1 mM AOAA. The distinct peaks of absorbance at 420 and 340 nm were not observed in the presence of buffer alone or when BSA was used instead of serine racemase.
