TABLE 2.

iNOS, NO, and GSH levels in J774 cells^a

Infection and treatment	Nitrite production (nmol/mg of protein)b		Pc	iNOS level (% fluorescent)d	GSH level (nmol/mg of protein)e		Pc
	Mean	SE			Mean	SE
No infection	0.162	0.034		2.4	3.99	0.284
BCG
None	0.128	0.020		11.06	4.127	0.068
IFN-γ + LPS	1.889	0.147	0.003	26.22	0.434	0.041	0.0127f
DPP
None	0.136	0.018		6.82	3.93	0.585
IFN-γ + LPS	2.241	0.041	0.0001	24.15	0.748	0.100	0.033f

^aJ774.1 cells were maintained in DMEM containing 10% fetal bovine serum. Macrophages were infected with mycobacteria at a 10:1 ratio and incubated for 2 h. Unphagocytosed organisms were removed by washing, and the infected macrophages were maintained in DMEM containing 10% fetal bovine serum or in medium containing IFN-γ (100 U/ml) and LPS (1 μg/ml). Infected macrophage cultures were terminated at 72 h to determine nitrite production and iNOS and GSH levels.

^bDetermined by the Griess reaction.

^cCalculated with the Statview program.

^diNOS levels were studied by immunocytochemical staining and analysed by flow cytometry.

^eDetermined by spectrophotometry.

^fCompared with the value for untreated cells.