TABLE 5.

Growth of BCG in cytokine (IFN-γ and LPS)- and BSO-treated J774.1 macrophages^a

Treatment	Colony countb				Pc
	Mean		SE
	1 h	72 h	1 h	72 h
None	52,400	64,800	6,539	1,600	0.1393
IFN-γ + LPS	44,800	20,066	3,555	2,034	0.0038
IFN-γ + LPS + BSO	62,000	80,266	1,006	3,235.9	0.0057

^aJ774.1 cells were maintained in DMEM containing 10% FBS and then infected with BCG at an MOI of 10:1. At 2 h after infection, unphagocytosed organisms were removed. BCG-infected macrophages were maintained in DMEM containing 10% fetal bovine serum, containing 10% fetal bovine serum and IFN-γ (100 U/ml) plus LPS (1 μg/ml), or containing 10% fetal bovine serum, IFN-γ (100 U/ml), LPS (1 μg/ml), and BSO (500 μM).

^bIntracellular colony counts were determined by plating lysed cultures on Middlebrook 7H11.

^cCalculated with the Statview program.