TABLE 7.
Survival of BCG and DPP in HMDMa
| Bacterial strain and treatment | Colony countb
|
Pc | |||
|---|---|---|---|---|---|
| Mean
|
SE
|
||||
| 1 h | 7 days | 1 h | 7 days | ||
| BCG | |||||
| None | 2,431 | 4,215 | 587.8 | 1,224 | 0.18 |
| IFN-γ + LPS | 4,297 | 6,550 | 103.8 | 116.4 | 0.42 |
| DPP | |||||
| None | 2,367 | 2,019 | 379.5 | 317.1 | 0.489 |
| IFN-γ + LPS | 3,040 | 3,076 | 44.66 | 49 | 0.965 |
a
Human monocytes from peripheral blood were maintained in vitro in RPMI containing 5% AB serum for 7 days for differentiation to macrophages. HMDM were infected with BCG or DPP at an MOI of 10:1 and maintained in media with or without IFN-γ and LPS (100 U/ml and 1 μg/ml, respectively), as described in Materials and Methods.
b
Intracellular colony counts were determined by plating lysed cultures on Middlebrook 7H11. Data are averages from five experiments done in triplicate.
c
Calculated with the Statview program.