TABLE 3.
IFN-γ secretion by T-cell line C2 (HLA-DR7,53) in response to MPB70 and its peptides in the presence of HLA class II-typed APC
| APC | ΔIFN-γ (102 pg/ml) in response toa:
|
||
|---|---|---|---|
| MPB70 mixture | Peptide p12 | Peptide p13 | |
| HLA-DR7,53 | 25.5 | 15.7 | 30.2 |
| HLA-DR4,53 | 51.6 | 25.4 | 43 |
| HLA-DR5,52,53 | 36 | 18.1 | 20.7 |
| HLA-DR5,7,52,53 | 33.8 | 22.7 | 21.7 |
| HLA-DR6,7,52 | <5 | <5 | <5 |
| HLA-DR3,7,52,53 | 35.2 | 35.6 | 22.8 |
a
In IFN-γ assays a response was considered positive if the IFN-γ concentration in a culture stimulated with antigen or peptide minus the IFN-γ concentration in a culture without antigen or peptide (ΔIFN-γ) was ≥500 pg/ml. Positive responses are indicated by boldface type. For the MPB70 mixture, peptide p12, and peptide p13 the HLA-DR restriction was DR53.