Skip to main content
. 2003 Apr;71(4):2047–2057. doi: 10.1128/IAI.71.4.2047-2057.2003

FIG. 2.

FIG. 2.

Soluble factors released by T. gondii-infected astrocytes enhance IL-10 secretion by microglia, which is correlated with the NO down-modulation mechanism. Microglial cells were incubated for 24 h in the presence of CMi or treated with IFN-γ (500 U/ml) in the presence of fresh medium, CMi, or CMc. The treatment with RP-8-Br cAMP (10 mM) or with anti-IL-10 (α-IL-10) neutralizing antibodies (10 μg/ml) was performed simultaneously with IFN-γ activation and CMi treatment. (A) Supernatants from microglia cells were collected and tested for IL-10 by enzyme-linked immunosorbent assay. The data represent the means + standard deviations (error bars) of three independent experiments. (B) Supernatants were collected and tested for nitrite using the Griess reaction. The data represent one of three independent experiments. *, P < 0.05.