FIG. 2.

Responses of memory and naive CD4⁺ T cells after stimulation with MP or TT. (A) CD4⁺ cells were labeled with anti-CD45RA-FITC and anti-L-selectin-PE antibodies and sorted into memory (CD45RA⁻ L-selectin^+/− or CD45RA⁺ L-selectin⁻) and naive (CD45RA⁺ L-selectin⁺) populations. The contour plots show 1 representative experiment out of 22. The percentages of memory and naive cells are shown inside the gates. (B to E) Memory and naive cells were stimulated with DCs pulsed with MP (B and C) or TT (D and E), and the resulting proliferation and IFN-γ production were determined. Each symbol represents the response of one individual after subtraction of the background response. Median values are indicated by horizontal bars. The Mann-Whitney test was used for statistical evaluation. n.s., not significant.