TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| Y. enterocolitica | ||
| WA-314 | Y. enterocolitica O:8 biotype 1B with pYV virulence plasmid | 25 |
| WA-C | Plasmidless derivative of strain WA-314; spontaneously resistant to nalidixic acid | 25 |
| WA-CS | Derivative of WA-C; spontaneous Smr mutant | This study |
| 8081 | Clinical isolate, O:8 biotype 1B | 40 |
| WA-CS yts1E::Kanr | Derivative of WA-CS; Nalr, Kanr Smr (insertional inactivation of yts1E by a kanamycin cassette) | This study |
| Ye1209-79 | Clinical isolate, O:13 biotype 1B | 25 |
| Ye1223-75-1 | Clinical isolate, O:20 biotype 1B | 25 |
| Ye737 | Clinical isolate, O:21 biotype 1B | 25 |
| Y-108 | Clinical isolate, O:3 biotype 4 | 25 |
| Y-96 | Clinical isolate, O:9 biotype 2 | 25 |
| NF-O | Clinical isolate, O:5 biotype 1A | 42 |
| MRS-40 | Clinical isolate, O:9 biotype 2 | 25 |
| Y. pseudotuberculosis | ||
| H141/84 | Serotype O:1a | S. Aleksic |
| H457/86 | Serotype O:2a | S. Aleksic |
| IP3295 | Serotype O:1 | E. Carniel |
| 346 | Serotype O:3 | S. Aleksic |
| Y. pestis | ||
| KIM | Biotype Mediaevalis | R. R. Brubaker |
| KUMA | Biotype Antiqua | R. R. Brubaker |
| TS | Biotype Orientalis | R. R. Brubaker |
| E. coli | ||
| C-4441 | Enteroaggregative, O:128 | 47 |
| 12860 | Enteroinvasive, O:124 | 47 |
| DH5α | endA1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 (φ80lacZΔM15) | 23 |
| S17-1λpir | pir+tra+ | 48 |
| XL1-Blue MR | Δ(mcrA) 183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lacc | Stratagene |
| MOSBlue | endA1 hsdR17 (rK12− mK12+) supE44 thi-1recA1 gyrA96 relA1 lac [F′ proA+B+ lacIqZΔM15:Tn10(Tcr)] | Amersham |
| Plasmids | ||
| Supercos1 | Cloning vector; Apr Neor | Stratagene |
| ScF yts1E::Kanr | BglII/BamHI self-religated Supercos1 fragment carrying the mutated yts1E gene | This study |
| pKAS 32 | Cloning vector with dominant rpsL gene | 49 |
| pKAS 32 yts1E::Kanr | pKAS32 carrying a fragment from the putative type II yts1E gene inactivated by a kanamycin cassette | This study |
| pACYC184 | Cloning vector; Cmr Tcr | New England Biolabs |
| pUC4K | pUC vector carrying the kanamycin cassette from Tn903; Kanr Apr | Amersham |
| pMOSBlue | Cloning vector; Apr | Amersham |
a
Nalr, nalidixic acid resistant; Tcr, tetracycline resistant; Cmr, chloramphenicol resistant.