TABLE 1.

Primers and plasmids

Primer	Sequence (5′-3′)a	Purpose or plasmid constructionb
F2Es	GAGCAGATCTATGACAGAGCAGCAGTGGAATTTC	pISM417
R1Es	GGCAGATCTCTATGCGAACATCCCAGTG	pISM417
R2Es	GTTGGATCCTGCGAACATCCCAGTGACG	pISM418
FP71.S1	GGGACAACAACAGAAAACTGGCTTTACC	P71 mutagenesis, fragment A
RP71.S1	GGTAAAGCCAGTTTTCTGTTGTTGTCCC	P71 mutagenesis, fragment A
FP71.S2	GAATTTAAAAAATGGGCAAGTATAATAAAACC	P71 mutagenesis, fragment B
RP71.S2	GGTTTTATTATACTTGCCCATTTTTTAAATTC	P71 mutagenesis, fragment B
FP71.S3	GATGTCTATGATAAAAGTATTTGGAATGATTCTG	P71 mutagenesis, fragment C
RP71.S3	CAGAATCATTCCAAATACTTTTATCATAGACATC	P71 mutagenesis, fragment C
FM71.S3	TGAAAGGATCCTGATAAAAATTTAATGGCGAA	P71 mutagenesis, pISM409
RM71.S3	ATCAGGATCCTTCCAAATACTTTTATCATAGAC	P71 mutagenesis, pISM409
P71.1	GCTAGATCTTTGCAAAAAAATTCTTTGCTTTC	pISM421
P71.2	GCTAGATCTTTAGT TTGATTTAGGCTCGGTAC	pISM421

^aRestriction sites introduced for cloning are in bold.

^bPrimers were used in the indicated pISM plasmid constructions.