FIG. 2.
Impact of vector priming in Nramp1+/+ mice. Control and vector-primed BALB/c (▪) and CBA (□) animals were dosed with serovar Stanley-K88 on day 0. Blood and fecal pellet samples were collected at intervals, and serum IgG (A and B) and gut IgA responses (C and D) were determined by ELISA. The latter are expressed as antibody titer per milligram of IgA to correct for varying immunoglobulin content. Graphs show log10 anti-K88 (A and C) and anti-LPS (B and D) ELISA titers (geometric mean ± standard deviation; n = 5 at each time point) in control (solid lines) and vector-primed (broken lines) animals. Broken lines in panels A and B show the limit of detection of serum ELISAs at 1 in 20.