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Immunoblot analysis of fractions eluted from the His-CesD2 capture column. (a) Fractions 2, 3, and 4 (lanes 1, 2, and 3, respectively) probed with polyclonal anti-His sera. (c) The same fractions, probed with monoclonal anti-EspD antibody, demonstrating that the secreted form of EspD copurifies with His-CesD2. (b and d) Control, His-T7 column fractions 2, 3, and 4, probed with anti-His polyclonal sera and monoclonal anti-EspD antibody, respectively, showing no copurification.
