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. 2003 Apr;71(4):2130–2141. doi: 10.1128/IAI.71.4.2130-2141.2003

FIG. 3.

FIG. 3.

Immunoblot analysis of culture supernatants showing the EspD secretion profile of EPEC strains. Secretion of EspD is greatly reduced in ICC172 (lane 2) compared with the level of secreted EspD in the supernatant of the wild-type E2348/69 strain (lane 1). Complementation of ICC173 with pICC246 resulted in an increased level of secreted EspD, although not to the wild-type level. Blots were probed with monoclonal EspD antibodies and developed by alkaline phosphates (lane 1) or chemiluminescence (lanes 2 and 3).