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FAS tests. After 1 h the wild-type strain adhered to HEp-2 cells in small microcolonies and induced disorganized actin accumulation, whereas strain ICC172 showed reduced adhesion with little actin accumulation. By 3 h however, both strains showed good adhesion and both strains produced discrete foci of actin accumulation beneath adherent bacteria, i.e., produced a positive FAS reaction.
