TABLE 3.
Oligonucleotide primers used in this study
| Primer name (restriction site) | Sequencea |
|---|---|
| CesD2-F (NdeI) | 5′ TATCATATGGTCGATACGTTTAATGATG 3′ |
| CesD2-R (BamHI) | 5′ TATGGATCCTTAACTATTTACGTTCATTACGAAC 3′ |
| LEE-4K-F | 5′ GGTGAAGTTACGAGTCGAACAGAGG 3′ |
| LEE-4K-R | 5′ GGCTATACAGGAGCGGTTTGGTCTG 3′ |
| CesD2-INV-F (SmaI) | 5′CCGCCACCCGGGAAAAAAGGCACTGCCACAAAGAAACTCC 3′ |
| CesD2-INV-R (SmaI) | 5′AAGGGTCCCGGGCAGCAAGAAACAGAAGACGGAATTTGGTTC 3′ |
| CesD2CR(+1)-F (NcoI) | 5′ TATCCATGGTCGATACGTTTAATGACG 3′ |
| CesD2CR(+408)-R (SalI) | 5′ TATGTCGACCTAATTATTTACTTTCATTACATACC 3′ |
| CesD2CR(−400)-F | 5′ AATCGTACATCAGGTATCGCTGATG 3′ |
| CesD2CR(+808)-R | 5′ TCTGATAAGATTTGCGGCAATACATTCA 3′ |
| CesD2CR(−45)-F (BamHI) | 5′ TATGGATCCATTAATCGTATGGGGCAATCGGC 3′ |
| AphT-R | 5′ GCTTGATGGTCGGAAGACG 3′ |
| pACYC-F | 5′ TCACAGTTAAATTGCTAACGCAGTCAGGCA 3′ |
a
Restriction sites are underlined.