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SDS-PAGE and Western blot analyses of purified truncated MBP-FedF proteins. Fusion proteins were isolated from E. coli strains carrying pKTH5068, pKTH5069, pKTH5070, pKTH5071, pKTH5072, pKTH5073, pKTH5075, pKTH5076, pKTH5077, or pKTH5094 (lanes 1 to 10, respectively). MBP was used as a control (lane 11). (A) Proteins were run on an SDS-polyacrylamide gel and stained with Coomassie dye. (B) Immunoblots with the corresponding samples using an MBP antiserum.
