TABLE 2.
Oligonucleotides used
| Primer | Nucleotide sequence (5′→3′) | Usea |
|---|---|---|
| 508 | CACACAGGAAACAGCTATGAC | R-PCR for internal in-frame deletions of fedF |
| 614 | GTGGAATTCACAAACTCTACTACTGCAGTG | R-PCR for internal in-frame deletions of fedF |
| 615 | GCAAGCTTATGTGGTCTACTTATTACGCG | PCR |
| 815 | ATCTTGATCATTCCGTTACTCTTG | PCR |
| 816 | TCTATGATCACACAAGTCTGTTTCATACGACTGG | PCR |
| 817 | GGAATGATCACACTACCCAAATGCCAGC | PCR |
| 818 | CAATTGATCATTCATTCCTAGTTCATCAGG | PCR |
| 819 | TACGAAGCTTGATCTCAAAACCAGTAAATCG | PCR |
| 820 | CTGAAAGCTTATAATAAACAGGCGTAGCTTGG | PCR |
| 821 | TCATAAGCTTGCTCTTAGACAAATCATACC | PCR |
| 861 | GGAACTATTTTGCAATATGCGCTCGAGTGTCGTGTG | R-PCR for internal in-frame deletions of fedF |
| 862 | GAGCGCATATTGCAAAATAGTTCCAGCCTGGCATG | R-PCR for internal in-frame deletions of fedF |
| 863 | CGTGTGAGCATTCAGTGGGGGCAACAGTCACAAGTAGG | R-PCR for internal in-frame deletions of fedF |
| 864 | TTGCCCCCACTGAATGCTCACACGACACTCGAGCG | R-PCR for internal in-frame deletions of fedF |
| 865 | GGATTCGGTACATTTACTGGTTTTGAGATCTCTTTACG | R-PCR for internal in-frame deletions of fedF |
| 866 | AAAACCAGTAAATGTACCGAATCCTACTTGTGACTG | R-PCR for internal in-frame deletions of fedF |
| 908 | ATCTGGATCCTTCCGTTACTC | PCR |
| 909 | TGTCAAGCTTTATGATCAACGCAAACACAGACAAAGAAATC | PCR |
| 910 | ACTCTGATCATAGACAAGTCTGTTTCATACGACTGG | PCR |
| 911 | GGGATGATCAACACTACCCAAATGCCAGC | PCR |
| 912 | TACATGATCAATTTCATTCCTAGTTCATCAGG | PCR |
| 974 | TGTGGAATTCTCTACTCTACAAGTAGACAAG | PCR |
| 975 | CCTGTCTAGATATGTGGTCTACTTATTACGCG | PCR |
| 976 | GTCTGAATTCTACGACTGGATCAATTCTAGTGCG | PCR |
| 977 | CACTGAATTCATGCCAGCTCTGTATACGTGG | PCR |
| 978 | CAATGAATTCTTCATTCCTAGTTCATCAGG | PCR |
| 985 | GAATTCTAGATTAGATCTCAAAACCAGTAAATCG | PCR |
| 986 | GTGCTCTAGATTAATAATAAACAGGCGTAGCTTGG | PCR |
| 987 | CTGTTCTAGATTAGCTCTTAGACAAATCATACC | PCR |
| 1108 | TTCAGAATTCACTTTGACATGCCAGGCTGG | PCR |
| 1109 | GAATTCTAGATTAGATCTCAAAACCAGTAAATCG | PCR |
| 1110 | TTTGGTATGGGCAAATGGGC | R-PCR, mutation K72A |
| 1111 | GCCCATTTGCCCATACCAAA | R-PCR, mutation K72A |
| 1112 | AAATGGGGCCGAAACCCAATATG | R-PCR, mutation R75A |
| 1113 | CATATTGGGTTTCGGCCCCATTT | R-PCR, mutation R75A |
| 1114 | ATGGGCGCGCAACCCAATATG | R-PCR, mutation E76A |
| 1115 | CATATTGGGTTGCGCGCCCAT | R-PCR, mutation E76A |
| 1116 | ATATGCGCTCGCGTGTCGTGTGAG | R-PCR, mutation E82A |
| 1117 | CTCACACGACACGCGAGCGCATAT | R-PCR, mutation E82A |
| 1118 | GCTCGAGTGTGCTGTGAGCATTCA | R-PCR, mutation R84A |
| 1119 | TGAATGCTCACAGCACACTCGAGC | R-PCR, mutation R84A |
| 1120 | GTGTGAGCATTGCCCATAGTTCTGG | R-PCR, mutation H88A |
| 1121 | CCAGAACTATGGCCAATGCTCACAC | R-PCR, mutation H88A |
| 1122 | GAGCATTCACGCTAGTTCTGGCTC | R-PCR, mutation H89A |
| 1123 | GAGCCAGAACTAGCGTGAATGCTC | R-PCR, mutation H89A |
| 1124 | GCTCCATTAATGCATCTCAGTGGG | R-PCR, mutation E96A |
| 1125 | CCCACTGAGATGCATTAATGGAGC | R-PCR, mutation E96A |
| 1135 | GTGGAAGCTTCATAGACAACCCTTCGATGC | R-PCR |
| 1325 | GTGTGAGCATTGCCGCTAGTTCTGG | R-PCR, double mutation H(88, 89)A |
| 1326 | CCAGAACTAGCGGCAATGCTCACAC | R-PCR, double mutation H(88, 89)A |
a
R-PCR, recombinant PCR.