TABLE 2.
Transformation frequency of wild-type and ruvC mutant strains with chromosomal and plasmid DNA
| Strain | Transformation frequencya (% of parent frequency)
|
||
|---|---|---|---|
| Chromosomal DNA | Plasmid pCTB2Cat | Plasmid pHel2 | |
| G27 | 956 ± 660 (100) | 745 ± 78 (100) | 970 ± 400 (100) |
| G27ruvC::Km | 21 ± 18 (2) | 43 ± 38 (6) | 733 ± 321 (76) |
| SS1 | 16 ± 8 (100) | 58 ± 10 (100) | 0 |
| SS1ruvC::Km | 0.6 ± 0.4 (4) | 3 ± 1 (5) | 0 |
a
Number of resistant colonies (10−8) per microgram of DNA per recipient CFU. Data are means ± standard deviations for three experiments.