TABLE 1.
List of plasmids relevant to this study
| Plasmid | Gene or description | Source or reference |
|---|---|---|
| pCIP3 | URA5 | 27 |
| pPM8 | URA5 containing episomal cloning vector | 20 |
| pYCC329 | GAL7::GUS in pPM8 backbone | This study |
| pPM22 | Rescued plasmid from transformant T1 | This study |
| pPM33 | 0.5-kb NotI fragment in TOPO 2.1 | This study |
| pPM34a | 0.5-kb NotI fragment from pPM33 inserted into pPM22 | This study |
| pPM34 | 4.5-kb genomic DNA fragment containing the CCN1 gene in pCIP3 | This study |
| pPM35 | ccn1 carrying a deletion of the three nucleotides CAA (positions 859-861) | This study |
| pPM36 | ccn1 carrying a deletion as well as substitution mutations in the pPM34 backbone | This study |
| pSC3 | TEF::CCN1 (cDNA) | This study |
| pSC8 | GAL7p::CCN1 (cDNA) in pYCC329 backbone | This study |
| pSC9 | GAL7p::CLF1 in pYCC329 backbone | This study |